Emerging bulk RNASeq applications

 Significant innovations in next-generation sequencing techniques and bioinformatics tools have impacted our appreciation and understanding of RNA. Practical RNA sequencing (RNA-Seq) applications have evolved in conjunction with sequence technology and bioinformatic tools advances. 

Article published in Briefing in Bioinformatics journal, published by Oxford Press.  In this review, Thind. et. al. explained the various new emerging applications of bulk RNASeq. Many new things could be discovered from the data which is already available on public platforms.  (Reference of the article, "Demystifying emerging bulk RNA-Seq applications: the application and utility of bioinformatic methodology")

In most projects, bulk RNA-Seq data is used to measure gene expression patterns, isoform expression, alternative splicing and single-nucleotide polymorphisms. However, RNA-Seq holds far more hidden biological information including details of copy number alteration, microbial contamination, transposable elements, cell type (deconvolution) and the presence of neoantigens. Recent novel and advanced bioinformatic algorithms developed the capacity to retrieve this information from bulk RNA-Seq data, thus broadening its scope. The focus of this review is to comprehend the emerging bulk RNA-Seq-based analyses, emphasizing less familiar and underused applications. In doing so, we highlight the power of bulk RNA-Seq in providing biological insights.

#RNAseq, #RNA-Seq, #NGS,  #Next generation, #Sequencing